The introduction of this standard in 2004 is valid for all member states of the European Union. In particular this standard gives engine and automobile makers the ability to give warranties to those vehicles which run on Biodiesel. At present a limit of 5% FAME is allowed in the EN590 specification for mineral diesel. This 5% must conform to the EN14214 standard. Diesel Fuel Injection Equipment (FIE) Manufacturers like Bosch stress the importance of EN14214.
All road fuels are subject to strict quality controls which are vital to maintain standards and provide authorities with the ability to assess safety risks and environmental pollution. For example regular mineral diesel is subject to the EN 590 standard. In 1997 the European Committee for Standardization was given the task to develop a uniform standard for Fatty Acid Methyl Ester (FAME). The result was the EN14214 specification.
| EN 14214 - Property | Units | Lower Limit | Upper Limit | Test-Method |
| Ester content | % (m/m) | 96,5 | - | EN 14103 |
| Density at 15°C | kg/m³ | 860 | 900 | EN ISO 3675 / EN ISO 12185. |
| Viscosity at 40°C | mm²/s | 3,5 | 5,0 | EN ISO 3104 |
| Flash point | °C | > 101 | - | ISO 3679 |
| Sulfur content | mg/kg | - | 10 | - |
| Tar remnant (at 10% distillation remnant) | % (m/m) | - | 0,3 | EN ISO 10370 |
| Cetane number | - | 51,0 | - | EN ISO 5165 |
| Sulfated ash content | % (m/m) | - | 0,02 | ISO 3987 |
| Water content | mg/kg | - | 500 | EN ISO 12937 |
| Total contamination | mg/kg | - | 24 | EN 12662 |
| Copper band corrosion (3 hours at 50 °C) | rating | Class 1 | Class 1 | EN ISO 2160 |
| Cold filter plugging point (CFPP) | °C | - | * | EN 116 |
| Oxidation stability, 110°C | hours | 6 | - | EN 14112 |
| Acid value | mg KOH/g | - | 0,5 | EN 14104 |
| Iodine value | - | - | 120 | EN 14111 |
| Linolic Acid Methylester | % (m/m) | - | 12 | EN 14103 |
| Polyunsaturated (>= 4 Double bonds) Methylester | % (m/m) | - | 1 | - |
| Methanol content | % (m/m) | - | 0,2 | EN 14110 |
| Monoglyceride content | % (m/m) | - | 0,8 | EN 14105 |
| Diglyceride content | % (m/m) | - | 0,2 | EN 14105 |
| Triglyceride content | % (m/m) | - | 0,2 | EN 14105 |
| Free Glycerine | % (m/m) | - | 0,02 | EN 14105 / EN 14106 |
| Total Glycerine | % (m/m) | - | 0,25 | EN 14105 |
| Alkali Metals (Na+K) | mg/kg | - | 5 | EN 14108 / EN 14109 |
| Phosphorus content | mg/kg | - | 10 | EN 14107 |